Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 WK6mutS(λ) (LMBP 1466)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | WK6mutS(λ) |
| Accession number: | LMBP 1466 |
| Depositor: | Prof. Dr E. Remaut1 2 1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 28°C (Note: 37°C is also possible but phage lambda remains more stably integrated when grown at 28°C) |
| Genotype: | F' lacIq Δ(lacZ)M15 proA+B+ traD36/ Δ(lac-proB) galE rpsL mutS::Tn10 λ |
| Phenotype: | StrR TetR |
| Properties: | Useful strain for site-directed mutagenesis; disabled mismatch repair due to knocked-out mutS. The Tn10 insertion renders the strain tetR. |
| Further information: | Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations. |
| Original reference: | Zell et al., EMBO J. 6 (1987), 1809-1815 [PMID: 3038536] |
| Related reference: | Stanssens et al., Nucleic Acids Res. 17 (1989), 4441-4454 [PMID: 2501754] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 WK6mutS(λ) (LMBP 1466) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Zell et al., 1987. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.